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Develop Dyes

NIRvana Sciences seeks to develop novel dyes that advance the field of multiplexing.

We define multiplexing as a type of test that simultaneously measures many color-labeled compounds in a single instrumentation run or cycle of test.  In a life sciences context, this can be described as a multiplex assay that measures multiple analytes (8 or more) in a single run.  An example of multiplexing can be found in a popular medical diagnostics platform called flow cytometry which is projected to exceed $4 billion by 2015.

As researchers and clinicians strive to extract more data from their flow cytometers, they seek to analyze more and more biological material in each run. A key constraint to more multiplexing is the spectral overlap created by the dyes used to color label their reagents.

dyes_1_f1

Figure 1 shows 5 dyes excited by a single laser in a flow cytometer. See that Dye 4 overlaps with the other four dyes and that most overlap with one another. This spectral overlap (spillover) needs to be compensated (deconvuluted).

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Figure 2 shows 5 dyes excited by a single laser in a flow cytometer; however, their emission spectra are ultra narrow causing very little spectral overlap .

•This provides significant user benefits including:

…….-Cleaner and better data
…….-Saves significant time  and money by reducing spectral complexities
…….-Quickly move into greater multiplexing applications because managing the complexities associated with
…….-spectral overlap requires a tremendous learning curve along with the associated complex experimental
…….-controls and safeguards which currently required an advanced set of skills

NIRvana Sciences bacteriochlorins offer the preferred multiplexing emission spectra like Figure 2.